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PMCID: PMC7120747

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You will also need alcohol swabs for disinfection, bacteriostatic water for reconstitution (if using lyophilized powder), a sterile mixing syringe if reconstituting, and a sharps container for safe needle disposal

Supernatant was taken from HepG2 and HepG2 ATP7B-KO cells treated with CuCl 2 (0, 0.2, 0.6mmol/l) for 24 h